Application

This Anti-CD22 Antibody, clone CY34 is validated for use in Flow Cytometry and Immunoprecipitation for the detection of CD22.

Research Sub CategoryImmunoglobulins & Immunology

Research CategoryInflammation & Immunology

Flow Cytometry Analysis: A representative lot detected the exogenously expressed mCD22, but not mCD40, on the surface of respective BHK transfectants (Law, C.L., et al. (1993). J. Immunol. 151(1):175-187).
Flow Cytometry Analysis: A representative lot selectively detected the Lyb-8.2 allelic form of Cd22 on sIgM-positive B lymphocytes from BALB/c mice, but not the Lyb-8.1 allelic form of Cd22 expressed on B lymophocytes from DBA/2J mice (Law, C.L., et al. (1993). J. Immunol. 151(1):175-187).
Immunoprecipitation Analysis: A representative lot immunoprecipitated the Lyb-8.2 allelic form of Cd22 from the splenocytes of B10.D2 mouse strain (Symington F.W., et al. (1982). Immunogenetics. 16(5):381-391).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

B-cell receptor CD22 (UniProt P20273; also known as B-lymphocyte cell adhesion molecule, BL-CAM, Sialic acid-binding Ig-like lectin 2, Siglec-2, T-cell surface antigen Leu-14) is encoded by the Cd22 (also known as A530093D23, Lyb-8, Lyb-8a, Lyb8, Siglec2) gene (Gene ID 12483) in murine species. Murine CD22 (mCD22) is a B cell-associated adhesion protein with seven extracellular Ig-like domains that has 62% amino acid homology to its human orthologue. CD22 is expressed at multiple stages during B cell ontogeny. Cytoplasmic hCD22 can be detected at the pro-B and pre-B, whereas expression of surface hCD22 tightly correlates with that of surface immunoglobulins (sIg), especially that of sIgD. Expression of hCD22 ceases upon B cell activation and terminal differentiation into plasma cells. Only the CD22+ subpopulation among sIgM+ tonsillar B cells can be stimulated by sIg cross-linking to induce intracellular calcium, which can be further enhanced by anti-CD22 treatment prior to sIg cross-linking. Crosstalk between CD22 and sIgM is also supported by the finding that CD22 can associate with sIgM and cross-linking of sIg can stimulate rapid tyrosine phosphorylation of CD22.

Immunogen

Splenocytes from mouse strain B10.D2

Epitope: Extracellular Ig-like domains 1 & 2

Other Notes

Concentration: Please refer to lot specific datasheet.

Physical form

Purified mouse monoclonal IgG1κ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Flow Cytometry in splenocytes from mouse strain C57BL/6.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD22 on splenocytes from mouse strain C57BL/6.

Specificity

Murine Cd22 gene is also known as Lyb-8. The epitope detected by clone CY34 is not expressed by all strains of mice due to Cd22 polymorphisms. Clone CY34 is known to react with Cd22 expressed on splenocytes from murine strains B10.D2 and BALB/c, C57BL/6, but not DBA/2J. Consequently, the allelic form of Cd22 from DBA/2J is designated as mCD22.1/Lyb-8.1 to distinquish it from the form of Cd22 recognized by clone CY34, originally designated as Lyb-8.2 (PMID 8100843 & 6984010).

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~95 kDa calculated